Cell-type-specific H+-ATPase activity and antioxidant enzymes improve the Echinacea purpurea L. Moench tolerance to salinity stress at different NO3-/NH4+ ratios

While the importance of biochemical processes in plant adaptive responses is widely accepted, only a limited number studies have addressed cell-type specific and enzymatic activities E. purpurea under salt stress conditions. So, various antioxidant activities, H+ transport activity, non-invasive ion flux mechanisms root apex mature zones grown at different NO3-/NH4+ ratios (90:10, 80:20, 70:30) were studied salinity condition (0, 15, 30, 60 mM NaCl). Various parameters, photosynthetic transpiration rates, gas exchange, accumulation, electrolyte leakage (EL) addition to enzyme cell-specific measured according standard methods. Based on results, NaCl significantly decomposed cell membrane, causing increase malondialdehyde (MDA) content (89.4% compared with control) decrease rate (84% control). The activity enzymes such as superoxide dismutase (SOD) ascorbate peroxidase (APX), catalase (CAT), glutathione (GP) increased up 30 diminish free radicals by increasing hydrogen peroxide (H2O2) concentration High Na+ Cl- their accumulation leaves caused inhibit antioxidants stress. nitrogen (N) supply form, more physiological properties found 90:10 ratio. Salinity limit growth high tissue- treatment-specific manner. differential sensitivity between two originates from 10-fold difference K+ efflux H+-ATPase zone apical region.